April 6, Tue 2010
1:00 pm, MRB 200 Conference Room
Dr. Marcella McClure
Dept of Microbiology and Center for Computational Biology, Montana State University
A Bioinformatic Approach to the Structure, Function, and Evolution of The Nucleoprotein Of The Order Mononegavirales
The goal of this Bioinformatic study is to investigate sequence conservation in relation to evolutionary function/structure of the nucleoprotein of the order Mononegavirales. In the combined analysis of 63 representative nucleoprotein sequences from the four viral families we predict the regions of protein disorder, intra-residue contact and co-evolving residues. Correlations between location and conservation of predicted regions illustrate conserved regions among the nucleoproteins and reflect an evolutionary advantage in maintaining these sites for the viral nucleoprotein as part of the transcription/replication machinery. The similarity in this protein among Mononegavirales in the C-terminus region of the representative protein is specifically important for interacting with the phosphoprotein as crystal structure information correlates with these predictions.